Forms of gene editing technology include:
1. Homologous Recombination
Homologous recombination was the first method used for editing. Cellular technological approaches. genomes. Homologous recombination is the exchange (recombination) of genetic information between two similar (homologous) strands of DNA.
2. Nucleases
The key to gene editing is creating DSBs at specific sites in the genome. Commonly used restriction enzymes are effective at cutting DNA, but they often recognize and cut at multiple sites and have low specificity. To overcome this problem and create site specific DSBs.
Applications of gene editing technology:
The combination of gene editing and reproductive technologies such as in vitro bovine embryo culture allows the use of highly synthetic endonucleasesspecific directly into fertilized oocytes. Perform genome editing.
CRISPR
-Cas9 further increases the application scope of gene editing in targeted modification of animal genes. CRISPR-Cas9 enables single simultaneous knockout (KO) of multiple targets in fertilized mammalian eggs via direct cytoplasmic injection.
Single-cell gene expression analysis has resolved the transcriptional roadmap of human development, identifying key candidate genes for functional studies. Using genome-wide transcriptomic data to guide experiments, CRISPR-based genome editing tools make it possible to interfere with or delete key genes to elucidate their functions.
Reference for the above content:Baidu Encyclopedia—Gene Editing Technology