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International
Standard
ISO 5667-3
Sixth edition
Water quality — Sampling —
2024-03
Part 3:
Preservation and handling of
water samples
Qualité de l'eau — Échantillonnage —
Partie 3: Conservation et manipulation des échantillons d'eau
Reference number
© ISO 2024
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Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms for plastics . 2
5 Sampling and chain of custody . 3
6 Reagents and materials . 3
6.1 Solids .3
6.2 Solutions .4
6.3 Materials .5
7 Containers . 5
7.1 Container selection and preparation .5
7.2 On-site filtration . .6
7.3 Filling the container .6
8 Sample handling and preservation . 6
8.1 General .6
8.2 Sample handling and preservation for physical and chemical analysis .7
8.3 Sample handling and preservation for hydrobiological analysis .7
8.4 Sample handling and preservation for radiochemical analysis .8
9 Sample transport . 8
10 Identification of samples . 9
11 Sample reception . 9
12 Sample storage . 9
Annex A (informative) Techniques for sample preservation .11
Annex B (informative) Container preparation .58
Bibliography .59
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 6,
Sampling (general methods), in collaboration with the European Committee for Standardization (CEN)
Technical Committee CEN/TC 230, Water analysis, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
This sixth edition cancels and replaces the fifth edition (ISO 5667-3:2018), which has been technically
revised.
The main changes are as follows:
— ISO/TS 5667-25 has been added as a reference;
— a flow diagram for preservation and storage of water samples has been added;
— references in Table A.1 have been updated;
— references in Tables A.2 and A.3 have been added;
— the previous Table A.1 has been split into Table A.1 on inorganic analytes and Table A.2 on organic
analytes;
— Table A.4 on microbiological analysis has been added;
— types of water have been added to Tables A.1 to A.5;
— the added terms used in Tables A.1 to A.5 have been explained.
A list of all parts in the ISO 5667 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body.
A complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
This document is intended to be used in conjunction with ISO 5667-1, which deals with the design of sampling
programmes and sampling techniques.
Where possible, this document has been aligned with current standards. Where new research or validation
results have provided new insights, the latest knowledge has been used.
[87]
Guidance on validation protocols can be found in ISO/TS 5667-25 and ISO 17034 .
Tables A.1 to A.5 provide the validated preservation times or conditions as well as the descriptions of best
practice. Tables A.1 to A.5 also refer, for each analyte, to references available at the time of publication of this
document (i.e. ISO 5667-3:2024). This is however not an exhaustive list. Other preservation methods may be
used when they have been validated. However, it is strongly recommended that, where a method validation
is not available, the preservation times for the analyte listed in Tables A.1 to A.5 for ISO test methods be
followed. In case more than one storage time is provided in Tables A.1 to A.5, the order of preferred use is:
— validated method;
— method provided by reference;
— best practice.
The preservation and storage conditions and maximum storage times per analyte as listed in Tables A.1 to
A.5 should be regarded as default conditions to be applied in the absence of any other information.
However, if validation of preservation techniques and holding times has been carried out, relative to specific
circumstances and matrices, by a laboratory, then, provided that it can produce evidence of this validation
where they differ from those set out in Tables A.1 to A.5, these validated preservation and storage conditions
and maximum storage times are deemed acceptable for use by the validating laboratories. A national
standard can contain information on preservation.
This document and the related analytical references can be used as presented in Figure 1.
v
WARNING — ‘Method provided by reference’ and ‘validated method’ can be based on previous standards
and methods and therefore not be in line with ISO/TS 5667-25. This information can be interpreted by
a qualified and experienced person.
Figure 1 — Flow diagram for the selection of a method for the preservation and storage of water samples
Attention is drawn to ISO/TS 5667-25, which contains guidelines and the elaboration of the required
techniques of how to validate new storage times or preservative methods and details of the techniques
described.
vi
International Standard ISO 5667-3:2024(en)
Water quality — Sampling —
Part 3:
Preservation and handling of water samples
1 Scope
This document specifies the general requirements for sampling, preservation, handling, transport
and storage of all water samples for physicochemical, chemical, hydrobiological and microbiological analyses
and determination of radiochemical analytes and activities.
Guidance on the validation of storage times of water samples is provided in ISO/TS 5667-25.
This document is not applicable to water samples intended for ecotoxicological assays, biological assays
(which is specified in ISO 5667-16), passive sampling (which is specified in ISO 5667-23) and microplastics
(which is specified in ISO 5667-27).
This document is particularly appropriate when samples cannot be analysed on site and have to be
transported to a laboratory for analysis.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 19458:2006, Water quality — Sampling for microbiological analysis
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
best practice
method based upon consensus or general use and that can be referred to in literature
Note 1 to entry: Given the differences in conditions and circumstances as well as the impossibility to
...