|
Specification
ISO/TS 5354-2
First edition
Molecular biomarkers — Detection
of DNA in cotton used for textile
2024-04
production —
Part 2:
Overview of target sequences for use
in polymerase chain reaction (PCR)-
based detection methods for cotton
genetically modified (GM) events
Biomarqueurs moléculaires — Détection d’ADN dans le coton
utilisé pour la production textile —
Partie 2: Présentation des séquences cibles à utiliser dans
les méthodes de détection reposant sur une réaction de
polymérisation en chaîne (PCR) des événements de coton
génétiquement modifié (GM)
Reference number
ISO/TS 5354-2:2024(en) © ISO 2024
---------------------- Page: 1 ----------------------
ISO/TS 5354-2:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: [email protected]
Website: www.iso.org
Published in Switzerland
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO/TS 5354-2:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 2
5 GM element screening . 2
5.1 Principle .2
5.2 Procedure .3
5.3 Primers and probes .3
5.4 Reference materials .6
5.5 GM cotton events .6
5.6 Validation .9
5.7 Interpretation and expression of results .9
5.8 Results . .9
5.9 Reporting .
...