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IS0
1 N T E R NAT1 ON AL ORGAN I ZATl O N FOR STAN DAR DI ZATl O N
IS0 RECOMMENDATION
R 605
PULSES
METHODS OF TEST
1st EDITION
August 1967
COPYRIGHT RESERVED
The copyright of IS0 Recommendations and IS0 Standards
belongs to IS0 Member Bodies. Reproduction of these
documents, in any country, may be authorized therefore only
of that country, being
by the national standards organization
a member of ISO.
For each individual country the only valid standard is the national standard of that country.
Printed in Switzerland
Also issued in French and Russian. Copies to be obtained through the national standards organizations.
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BRIEF HISTORY
The IS0 Recommendation R 605, Pulses - Methods of Test, was drawn up by Technical
Committee ISO/TC 34, Agricultural Food Products, the Secretariat of which is held by Office
Hongrois de Normalisation (MSZH).
Work on this question by the Technical Committee began in 1959 and led, in 1964, to the
adoption of a Draft IS0 Recommendation.
In June 1964, this Draft IS0 Recommendation (No.793) was circulated to all the IS0
Member Bodies for enquiry. It was approved, subject to a few modifications of an editorial
nature, by the following Member Bodies :
Y
Argentina Greece Romania
Australia Hungary Spain
Canada
India Switzerland
Chile Iran Turkey
Czechoslovakia Israel U.A.R.
Denmark Korea, Rep. of United Kingdom
France New Zealand U.S.S.R.
Germany
Poland
One Member Body opposed the approval of the Draft:
Netherlands.
The Draft IS0 Recommendation was then submitted by correspondence to the IS0
Council, which decided, in August 1967, to accept it as an IS0 RECOMMENDATION.
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ISO/R 605 - 1967 (E)
1 IS0 Recommendation R 605 August 1967 I
PULSES
METHODS OF TEST
1. SCOPE
This IS0 Recommendation describes methods for testing pulses which have not been processed
and are intended for human consumption or for animal feeding stuffs.
2. DETERMINATION OF IMPURITIES
2.1 Preparation of sample
Thoroughly mix the final lot sample obtained according to IS0 Recommendation R ,
Pulses - Sampling, * and reduce it if necessary, by means of an automatic divider or by hand,
to obtain the test portions specified in clause 2.2.
2.2 Test portions
The minimum test portion, for one determination, should be 200 g, except for butter beans
(Phaseolus Iunatus L.) and horse beans (Vicia faba L.) for which it should be 300 g.
If the content of impurities is very small, it may be necessary to increase considerably the
test portion.
2.3 Procedure
Separate the test portion into component groups in order to obtain information on the use
for which the lot is suitable.
Generally the test portion is separated into five groups, as follows:
(I) seeds typical of the species and variety;
(2) seeds typical of the species but of another variety;
(3) defective seeds belonging to the same species;
(4) organic impurities;
(5) inorganic impurities.
2.3.1 Seeds typical of the species and variety. This group includes all the intact sound seeds,
those with a cracked or injured seed-coat, seeds slightly infested by insects, and broken
seeds larger than one half their original size.
Each country is permitted to subdivide this group if desired.
2.3.2 Seeds typical of the species but of another variety. This group includes seeds of varieties
which differ significantly in shape, size, colour or appearance.
2.3.3 Defective seeds belonging to the same species. This group includes broken, bitten and
injured seeds equal to, or less than, one half their original size; seeds visibly damaged by
insects ; shrivelled, unripe, germinated seeds ; rotten, mouldy and diseased seeds.
2.3.4 Organic impurities. This group includes seed-coats, parts of stems, pods, leaves, etc.,
other crop seeds and weed seeds.
2.3.5 Inorganic impurities. This group includes earth, sand, dust, stones, etc.
* At present Draft IS0 Recommendation No. 1235.
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IÇO/R 605 - 1967 (E)
3. DETERMINATION OF SIZE
(of pulses intended for human consumption)
Carry out the determination of size on material falling within the groups described in clauses
2.3.1 and 2.3.2.
According to the species of pulse, use either sieves with round holes (for example, for peas and
lentils) or with suitable elongated holes (for example, for beans).
4. DETERMINATION OF THE MASS OF 1000 GRAINS
Carry out the determination as described in IS0 Recommendation R 520, Cereals and Pulses -
Determination of the Mass of IO00 Grains.
5. TESTS FOR PRESENCE OF FOREIGN ODOURS
5.1 This examination should be carried out as soon as possible after sampling, either on the
sample in its original condition or on the ground sample.
Spread the sample and smell it. If no strong foreign odour is observed, seal the sample again
for 24 hours and then check it anew. The sample may be examined also during the grinding
If after these operations no foreign odour can be detected with certainty, put
operation.
about 3 to 5 g of the ground seed into a flask of 50 to 100 ml capacity. Cautiously moving
the open flask over a flame or repeatedly shaking it in a water-bath, examine the sample at
a temperature not higher than 60 OC.
5.2 A rapid method (enhancing the development of the odour) is to put a small quantity of the
product in a beaker, pour in some warm water (60 to 70 OC), cover, after 2 or 3 minutes
decant the water and note whether foreign odours are present.
6. TESTS FOR INFESTATION BY INSECTS
The presence of insect pests, especially adults or larvae of the house moth type (e.g. Endrosis or
Hofmannophila species) or Bruchid species, either on sacks or inside the bulk, should be noted.
Examine for infestation by the X-ray method described in IS0 Recommendation R
, *
Cereals and Pulses - Method of Test for Infestation by X-ray Examination. If this is not possible,
use one of the methods described below, which are strictly qualitative.
(I) Test for visible infestation,
(2) Test for infestation by Bruchid beetles on peas by flotation,
(3) Test for infestation by Bruchid beetles on peas by a chemical procedure.
6.1 Test for visible infestation
Scatter part of the laboratory sample on a warm plate (about 40 OC) and cover immediately
with a bell jar in order to prevent the escape of the beetles. In warm climates it may be
advisable to cool the sample and then to sieve it quickly through a sieve of aperture
2 mm. Thus, the adult insects can easily be collected in a test tube and, if it is desired to know
whether living beetles are present, the closed test tube can be warmed for a few minutes by
hand.
If no living insects are observed within 15 minutes, open if possible 100 of the obviously
infested seeds to check the possible presence of living or dead beetles and larvae. Examine
the samples also for lace (filet) produced by the larvae of the house moth and related species.
* At present Draft IS0 Recommendation No. 1236.
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ISO/R 605 - 1967 (E]
I
6.2 Test for infestation by Bruchid beetles on peas by flotation
6.2.1 Preparation of salt solution. Use a solution of sodium chloride or of ammonium nitr
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