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IS0
I N T E RN AT I O N A L O R G A N IZ AT 1 ON FOR STAND AR D I ZATl O N
IS0 RECOMMENDATION
R 734
OILSEED RESIDUES
DETERMINATION OF OIL CONTENT
1 st EDITION
May 1968
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For each individual country the only valid standard is the national standard of that country.
Printed in Switzerland
Also issued in French and Russian. Copies to be obtained through the national standards organizations.
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BRIEF HISTORY
The IS0 Recommendation R 734, Oilseed residues - Determination of oil content, was drawn
up by Technical Committee ISO/TC 34, Agricultural food products, the Secretariat of which is held
by the Magyar Szabvanyugyi Hivatal (MSZH).
Work on this question by the Technical Committee began in 1963 and led, in 1965, to the
adoption of a Draft IS0 Recommendation.
In October 1966, this Draft IS0 Recommendation (No. 1041) was circulated to all the IS0
Member Bodies for enquiry. It was approved, subject to a few modifications of an editorial nature, by
the following Member Bodies :
Argentina Hungary Romania
Australia India South Africa,
Brazil Iran Rep. of
Bulgaria Israel Thailand
Czechoslovakia Italy Turkey
Chile Korea, Rep. of United Kingdom
Netherlands
Colombia U.S.S.R.
France Poland Yugoslavia
Germany Portugal
Two Member Bodies opposed the approval of the Draft :
Canada
Ireland
The Draft IS0 Recommendation was then submitted by correspondence to the IS0 Council,
which decided, in May 1968. to accept it as an IS0 RECOMMENDATION.
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ISO/R 734-1968 (E
IS0 Recommendation R 734 Mai 1968
OILSEED RESIDUES
DETERMINATION OF OIL CONTENT
INTRODUCTION
As the determination of the oil content of oleaginous seeds is carried out, according to IS0 Recom-
mendation R 659, Oleaginous seeds - Determination of oil content, by extraction with n-hexane or
light petroleum, it has been considered necessary that determination of the oil content of oilseed resi-
dues should also be carried out in the same way, so as to enable the oil industry to control manufact-
ure. This method is the subject of the present IS0 Recommendation.
However, the principal users of oilseed residues, namely, manufacturers of animal feeding stuffs, have
always carried out the determination by extraction with diethyl ether and have accumulated a large
amount of data on the subject. As the two methods do not always give the same results, it has been
considered necessary to establish a method using diethyl ether as well, and this forms the subject of
IS0 Recommendation R 736, Oilseed residues - Determination of diethyl ether extract.
1. SCOPE
This IS0 Recommendation describes a method for the determination of the oil content of residues
(excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or
solvent.
2. DEFINITION
By oil is meant the whole of the substances extracted under the operating conditions described below.
The oil content is expressed as a percentage by mass.
3. PRINCIPLE
Extraction of the oil from the product in a suitable apparatus, with a suitable solvent : n-hexane or,
fading this, light petroleum.
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ISO/R 734-1968 (E)
4. REAGENTS
4.1 n-Hexane or, failing this, light petroleum distilling between 40 and 60 "C and having a bromine
value less than 1. For either solvent, the residue on complete evaporation should not exceed
0.002 g/ 100 ml.
4.2 Sand, washed with hydrochloric acid and calcined.
4.3 Pumice stone, in small particles, previously dried.
5. APPARATUS
5.1 Analytical balance.
5.2 Mechanical mill, easy to clean and allowing the residue to be ground, without heating and with-
out appreciable change in the content of moisture, volatile matter and oil, to particles passing
completely through a sieve of aperture diameter 1 mm.
5.3 Sieve, with apertures of diameter 1 mm.
5.4 Extraction thimble or filter paper, and cotton wool, free from matter soluble in n-hexane or
light petroleum.
5.5 Suitable extraction apparatus, capacity of flask 200 to 250 ml, for example.
5.6 Electric heating bath, (sand bath, water bath, etc.) or hotplate.
5.7 Pestle and mortar, of porcelain, iron or bronze, or preferably a suitable mechanical micro-
grinder.
5.8 Elecîricallv heated oven, with temperature control.
5.9 Desiccator, containing an efficient desiccant.
6. PROCEDURE
6.1 Preparation of the sample
6.1.1 Use the contract sample obtained as described in IS0 Recommendation R .,* Oilseed resid-
ues - Sampling.
6.1.2 Grind the contract sample, if necessary, in the previously well cleaned mechanical mill (5.2).
Use about a twentieth of the sample to complete the cleaning of the mill, and reject these
grindings ; grind the rest, collect the grindings, mix carefully and carry out the analysis with-
out delay.
6.2 Test portion
6.2.1 As soon as grinding is completed, weigh to the nearest 0.01 g, about 10 g of the grindings
(6.1.2).
6.2.2 Put the test portion (6.2.1) into a thimble (5.4) and close the latter with a plug of cotton
wool (5.4). If a filter paper (5.4) is used, wrap the grindings in it.
*
At present at the stage of a draft proposal.
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ISO/R 734-1968 (E
6.3 Preliminary drying
If the residue
...