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SLOVENSKI STANDARD
01-marec-2023
Kakovost tal - Navodilo za izbiro in vrednotenje bioloških preskusov za
ekotoksikološko karakterizacijo tal in talnih materialov (ISO 17616:2019)
Soil quality - Guidance on the choice and evaluation of bioassays for ecotoxicological
characterization of soils and soil materials (ISO 17616:2019)
Bodenbeschaffenheit - Anleitung für die Auswahl und Beurteilung von Biotestverfahren
zur ökotoxikologischen Charakterisierung von Böden und Bodenmaterialien (ISO
17616:2019)
Qualité du sol - Lignes directrices relatives aux choix et à l'évaluation des essais
appliqués pour la caractérisation écotoxicologique des sols et des matériaux de type sol
(ISO 17616:2019)
Ta slovenski standard je istoveten z: EN ISO 17616:2022
ICS:
13.080.05 Preiskava tal na splošno Examination of soils in
general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 17616
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2022
EUROPÄISCHE NORM
ICS 13.080.05
English Version
Soil quality - Guidance on the choice and evaluation of
bioassays for ecotoxicological characterization of soils and
soil materials (ISO 17616:2019)
Qualité du sol - Lignes directrices relatives aux choix et Bodenbeschaffenheit - Anleitung für die Auswahl und
à l'évaluation des essais appliqués pour la Beurteilung von Biotestverfahren zur
caractérisation écotoxicologique des sols et des ökotoxikologischen Charakterisierung von Böden und
matériaux de type sol (ISO 17616:2019) Bodenmaterialien (ISO 17616:2019)
This European Standard was approved by CEN on 9 October 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17616:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 17616:2019 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 17616:2022 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2023, and conflicting national standards shall be
withdrawn at the latest by April 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 17616:2019 has been approved by CEN as EN ISO 17616:2022 without any modification.
INTERNATIONAL ISO
STANDARD 17616
Second edition
2019-10
Soil quality — Guidance on the choice
and evaluation of bioassays for
ecotoxicological characterization of
soils and soil materials
Qualité du sol — Lignes directrices relatives aux choix et
à l'évaluation des essais appliqués pour la caractérisation
écotoxicologique des sols et des matériaux de type sol
Reference number
ISO 17616:2019(E)
©
ISO 2019
ISO 17616:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
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below or ISO’s member body in the country of the requester.
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Published in Switzerland
ii © ISO 2019 – All rights reserved
ISO 17616:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
3.1 Assessment . 1
3.2 Types of soil and other soil materials . 2
3.3 Terms relating to Soil characteristics . 2
3.4 Land and sites . 3
4 Principles and applications of test batteries . 3
5 Testing strategy and interpretation of test results according to the use and re-use of
soils / soil materials and soil functions . 4
5.1 Monitoring of soil treatment success . 4
5.2 Assessment of the ecotoxic potential of soils / soil materials . 4
5.2.1 General. 4
5.2.2 Choice of test battery and evaluation of test results for assessing retention
function . 5
5.2.3 Choice of test battery and evaluation of test results for assessing habitat
function . 8
Annex A (informative) Tests with soil eluate — Expression of results .10
Bibliography .11
ISO 17616:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological characterization.
This second edition cancels and replaces the first edition (ISO 17616:2008), which has been technically
revised. The main changes compared to the previous edition are as follows:
[10]
— definitions for “soil use” and “ecosystem service” have been added to Clause 3;
— end points of ecotoxicity tests (e.g. mortality, reproduction, growth, genotoxicity, and other
functional activities), as well as the overall principles and application of test batteries have been
clarified in Clause 4;
— sub-chronic toxicity tests have been added;
— Figure 1 was revised;
— Tables 1 and 2 (test batteries for retention and habitat function assessment, respectively) have been
revised (test categories, test organisms added/deleted, references updated).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2019 – All rights reserved
ISO 17616:2019(E)
Introduction
The characterization of contaminated soils can be based on strategies considering chemical analyses
and/or bioassays. ISO 15799 provides guidance on the selection of experimental methods for the
assessment of the ecotoxic potential of soils and soil materials (e.g. excavated and remediated soils,
refills, embankments) with respect to their intended use or re-use, and possible adverse effects on
aquatic and soil dwelling organisms.
An assessment strategy giving instructions for the choice and evaluation of test results is hence
proposed. The evaluation of the bioassays outcome is based on empirically-derived critical dilution
levels that take into account the sensitivity of the test system and the intended use/re-use of the site
under investigation. This approach intends to contribute to an effective and comparable assessment
[1]
within the ecotoxicological characterization of contaminated soil or soil materials . The test systems
included in this approach are not mandatory and may be replaced or accomplished by other test methods.
Nevertheless, the selected test systems have proved to appropriately characterize contaminated soils
...