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International
Standard
ISO 7836
First edition
Natural rubber — Identification of
2024-07
phytosterols, including β-sitosterol
Caoutchouc naturel — Identification des phytostérols, en
particulier du β-sitostérol
Reference number
© ISO 2024
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Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 2
7 Procedure . 3
7.1 Sample preparation .3
7.2 Thin layer chromatography technique .3
7.2.1 Preparation of the TLC plates .3
7.2.2 Marking the TLC plates.3
7.2.3 Spotting the TLC plates .3
7.2.4 Developing the TLC plates.3
7.2.5 Identification of β-sitosterol .3
8 Expression of results . 4
9 Test report . 4
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Foreword
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This document was prepared by Technical Committee ISO/TC 45, Rubber and rubber products, Subcommittee
SC 3, Raw materials (including latex) for use in the rubber industry.
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International Standard ISO 7836:2024(en)
Natural rubber — Identification of phytosterols, including
β-sitosterol
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is
the responsibility of the user to establish appropriate safety and health practices prior to use.
1 Scope
This document specifies a method to identify the presence of phytosterol including β-sitosterol in natural
rubber in raw and vu
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