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IS0
IN TERN AT1 ON A L ORGAN IZ AT i O N FOR STAND AR D IZATl O N
IS0 RECOMMENDATION
R 736
OIL SEED RESIDUES
NAT
DETERM ON OF DIETHYL ETHER EXTRACT
1st EDITION
May 1968
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The copyright of IS0 Recommendations and IS0 Standards
belongs to IS0 Member Bodies. Reproduction of these
documents, in any country, may be authorized therefore only
by the national standards organization of that country, being
a member of ISO.
For each individual country the only valid standard is the national standard of that country.
Printed in Switzerland
Also issued in French and Russian. Copies to be obtained through the national standards organizations.
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BRIEF HISTORY
The IS0 Recommendation R 736, Oilseed residues - Determination of diethyl ether extract,
was drawn up by Technical Committee ISO/TC 34, Agriculrurol food products, the Secretariat of
which is held by the Magyar Szabvanyügyi Hivatal (MSZH).
Work on this question by the Technical Committee began in 1963 and led, in 1965, to the
adoption of a Draft IS0 Recommendation.
In October 1966, this Draft IS0 Recommendation (No. 1043) was circulated to all the IS0
Member Bodies for enquiry. It was approved, subject to a few modifications of an editorial nature, by
the following Member Bodies :
Australia India Romania
Bulgaria Iran South Africa,
Czechoslovakia Israel Rep. of
Chile Italy Thailand
Colombia Korea, Rep. of Turkey
France Netherlands U.A.R.
Poland United Kingdom
Gemiany
Hungary Portugal Yugoslavia
Two Member Bodies opposed the approval of the Draft :
Canada
Ireland
The Draft IS0 Recommendation was then submitted by correspondence to the IS0 Council,
which decided, in May 1968, to accept it as an IS0 RECOMMENDATION.
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ISO/R 736-1968 (E)
IS0 Recommendation R 736 May 1968
OIL SEED RESIDUES
DETERMINATION OF DIETHYL ETHER EXTRACT
INTRODUCTION
As the principal users of oiiseed residues, namely, the manufacturers of animal feeding stuffs, have
aiways determined the oil content of oilseed residues by extraction with diethyl ether and have
accumulated a large amount of data on the subject, it has been considered necessary to establish this
method of determination and this is the subject of the present IS0 Recommendation.
However, as the determination of the oil content of oiiseeds is carried out according to IS0 Recom-
mendation R 659, Oleaginous seeds - Determination of oil content, by extraction with n-hexane or
light petroleum, it has been thought necessary that the oil content of oiiseed residues should be
determined in the same way in order to provide for control of oil production. This method is the
subject of IS0 Recommendation R 734, Oilseed residues - Determination of oil content. The two
methods do not always give the same results.
1. SCOPE
This IS0 Recommendation describes a method for the determination of diethyl ether extract of
residues (excluding compounded products), obtained by the extraction of oil from oilseeds by pres-
sure or solvent.
2. DEFINITION
By diethyl ether extract is meant the whole of the substances extracted by this solvent under the
operating conditions described below.
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ISO/R 736-1968 (E)
3. PRiNCIPLE
Extraction of the product with diethyl ether in a suitable apparatus.
4. REAGENTS
Diethyl ether, anhydrous, practically free from peroxides (p 20 = 0.712 to 0.716 g/ml, boiling
4.1
point 34.5 OC), analytical quality, the non-volatile residue of which at 80 "C is not greater than
0.0019 'Io.
Sand, washed with hydrochloric acid and calcined.
4.2
4.3 Atmice stone, in small particles, previously dried.
4.4 Sodium sulphate, anhydrous, analytical quality.
5. APPARATUS
5.1 Analytical balance.
Mechanical mill, easy to clean and allowing the residues to be ground, without heating and
5.2
without appreciable change in the content of water, volatile matter and oil, to particles passing
completely through a sieve of aperture diameter 1 mm.
Sieve, with apertures of diameter 1 mm.
5.3
Extraction thimble, or fdter paper, and cotton wool, free from matter soluble in diethyl ether.
5.4
5.5 Suitable extraction apparatus, (capacity of flask 200 to 250 ml, for example).
5.6 Elecm'c heating bath, (sand bath, water bath, etc.).
5.7 Pestle and mortar, of porcelain, iron or bronze, or preferably, a suitable mechanical micro-
grinder.
5.8 Electrically heated vacuum oven, with temperature control.
5.9 Desiccator, containing an efficient desiccant.
6. PROCEDURE
6.1 Preparation of the sample
6.1.1 Use the contract sample obtained as described in IS0 Recommendation R . . .,* Oilseed
residues - Sampling.
6.1.2 Grind the contract sample, if necessary, in the previously well-cleaned mechanical miil
(5.2). Use about a twentieth of the sample to complete the cleaning of the mill, and reject
these grindings; grind the rest, collect the grindings, mix carefully and carry out the analysis
without delay.
At present at the stage of a draft proposal.
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ISO/R 736-1968 (E) '
6.2 Test portion
As soon as grinding is completed, weigh to the nearest 0.01 g about 5
...